An efficient synthesis of functionalized ß-amino- and ß-hydrazinoalkylphosphonates by three-component reaction between trialkyl phosphites, dialkyl acetylenedicarboxylates and aromatic amines or hydrazines.

Three-component reaction between trialkyl phosphites, dialkyl acetylenedicarboxylates and aromatic amines afforded ß-aminoalkylphosphonate derivatives. Similar reaction between trialkyl phosphites, dialkyl acetylenedicarboxylates and dinitrophenylhydrazine afforded ß-hydrazinooalkylphosphonate derivatives. This method includes both the C-N and C-P bond formation in a one pot and single synthetic step in neutral and simple reaction conditions. All reactions were conducted in CH2Cl2 as solvent at room temperature without using any catalyst, and the stable products were obtained in high yields. The structures of all products were proved by 1H, 13C and 31P NMR and IR spectral and elemental analysis data.

Survey reaction of 2-amino-N-(aryl) benzimidamides with ninhydrin in different conditions and investigation of reaction mechanism using density functional theory (DFT).

In this investigation, firstly, 1-(2-amino-phenyl)-N-(aryl) methane diamine derivatives were synthesized by reaction of 2-aminobenzo nitrile with aromatic amines in the presence of aluminum chloride as the catalyst. Then, the reaction of these intermediates with ninhydrin in different conditions was investigated. The reaction between ninhydrin and 2-amino-N'-(aryl) benzimidamide derivatives in water as solvent under reflux conditions resulted in the synthesis of diazepine derivatives. The same results were obtained when the reaction was done in EtOH and in the presence of a few drops of sulfuric acid at room temperature. Also, this reaction was carried out in ethanol as solvent without the presence of sulfuric acid at room temperature which resulted in the synthesis of spiro [indene-2,2'-quinazoline] derivatives. And finally, the reaction was carried out in ethanol as solvent without the presence of sulfuric acid at the reflux conditions which resulted in the synthesis of isoquinolino-quinazoline derivatives. These N-heterocycles compounds are important biologically. Mild reaction conditions, simple procedure and purification and also product diversity with changing conditions are important advantages of this method. Also, to better understanding reaction mechanism on the condensation reactions of 2-amino-N-(aryl) benzimidamides with ninhydrin in different conditions, density functional theory (DFT)-based quantum chemical methods have been applied. Calculated atomic charges suggest that the C-1 (+ 0.54 a.u.) center of ninhydrin is a better electrophile than C-2 (+ 0.42 a.u.) center.

Carbon fixation of CO2 via cyclic reactions with borane in gaseous atmosphere leading to formic acid (and metaboric acid); A potential energy surface (PES) study.

Carbon dioxide (CO2), a stable gaseous species, occupies the troposphere layer of the atmosphere. Following it, the environment gets warmer, and the ecosystem changes as a consequence of disrupting the natural order of our life. Due to this, in the present reasearch, the possibility of carbon fixation of CO2 by using borane was investigated. To conduct this, each of the probable reaction channels between borane and CO2 was investigated to find the fate of this species. The results indicate that among all the channels, the least energetic path for the reaction is reactant complex (RC) to TS (A-1) to Int (A-1) to TS (A-D) to formic acid (and further meta boric acid production from the transformation of boric acid). It shows that use of gaseous borane might lead to controlling these dangerous greenhouse gases which are threatening the present form of life on Earth, our beautiful, fragile home.

Six-component synthesis and biological activity of novel spiropyridoindolepyrrolidine derivatives: A combined experimental and theoretical investigation.

Petasites hybridus rhizome water extract was used as green media for the preparation of Ag/Fe3O4/CdO@multi-walled carbon nanotubes magnetic nanocomposites (Ag/Fe3O4/CdO@MWCNTs MNCs), and its activity was evaluated by using in the one-pot multicomponent reaction of isatins, acetyl chloride, secondary amines, vinilidene Meldrum's acid, primary amines, and malononitrile in an aqueous medium at room temperature for the generation of spiropyridoindolepyrrolidine as new derivatives with tremendous output. In addition, organic pollutant reduction of 4-nitrophenol (4-NP) was carried out by generated Ag/Fe3O4/CdO@MWCNTs in water at room temperature. The results displayed that Ag/Fe3O4/CdO@MWCNTs were reduced as pollutants of organic compounds in a short time. The synthesized spiropyridoindolepyrrolidine has an NH2 functional group that has acidic hydrogen and shows high antioxidant ability. Also, the spiropyridoindolepyrrolidine exhibited antimicrobial ability, and the method that is used for this purpose is the disk diffusion method, and two kinds of bacteria, Gram-positive and Gram-negative, were employed for this analysis. Also, to better understand the reaction mechanism density, functional theory-based quantum chemical methods have been applied. For the generation of spiropyridoindolepyrrolidine, the used process has many properties such as reactions with short time, product with good yields, and simple extraction of catalyst from the mixture of reaction.

Adsorption of Carbon Dioxide with Ni-MOF-74 and MWCNT Incorporated Poly Acrylonitrile Nanofibers.

Among the new adsorbent forms, nanofiber structures have attracted extra attention because of features such as high surface area, controllable properties, and fast kinetics. The objective of this study is to produce the polyacrylonitrile (PAN) electrospun nanofibers loaded with Ni-MOF-74/MWCNT to obtain maximum CO2 adsorption. The prepared PAN/MWCNT/MOF nanofiber based on the Box-Behnken design (BBD) model suggests the CO2 adsorption of about 1.68 mmol/g (at 25 °C and 7 bar) includes 14.61 w/v%, 1.43 w/w%, and 11.9 w/w% for PAN, MWCNT, and MOF, respectively. The results showed the effective CO2 adsorption of about 1.65 ± 0.03 mmol/g (BET = 65 m2/g, pore volume = 0.08 cm3/g), which proves the logical outcomes of the chosen model. The prepared PAN/MWCNT/MOF nanofiber was characterized using different analyzes such as SEM, TEM, TG, XRD, FTIR, and N2 adsorption-desorption isotherms. More MOF mass loading on the nanofiber surface via secondary growth method resulted in 2.83 mmol/g (BET = 353 m2/g, pore volume = 0.22 cm3/g, 43% MOF mass loading) and 4.35 mmol/g (BET = 493 m2/g, pore volume = 0.27 cm3/g, 65% MOF mass loading) CO2 adsorption at 7 bar for the first and second growth cycles, respectively. This indicates that secondary growth is more effective in the MOF loading amount and, consequently, adsorption capacity compared to the MOF loading during electrospinning.

Design and characterization of keratin/PVA-PLA nanofibers containing hybrids of nanofibrillated chitosan/ZnO nanoparticles.

In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated. PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector. Addition of CHNF and ZnONPs amounts in CSZ combination resulted in reduction of the diameter of nanofibers. The highest hydrophilicity was reported for K/PVA/CS-PLA/CS sample with the contact angle of about 43 ± 3°. AFM results for K/PVA-PLA, K/PVA/CS-PLA/CS and K/PVA/CSZ(2:1)-PLA/CSZ(2:1), K/PVA/CSZ(1:2)-PLA/CSZ(1:2) samples indicated that the surface roughness factor for these nanofibers was about 708, 277, 378 and 658 nm, respectively. DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures. Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.

Efficient co-cultivation of human fibroblast cells (HFCs) and adipose-derived stem cells (ADSs) on gelatin/PLCL nanofiber.

In this study, we investigated whether the nanofibers produced by natural-synthetic polymers can probably promote the proliferation of co-cultured adipose-derived stem cells/human fibroblast cells (ADSs/HFCs) and synthesis of collagen. Nanofiber was fabricated by blending gelatin and poly (L-lactide co-ɛ-caprolactone) (PLCL) polymer nanofiber (Gel/PLCL). Cell morphology and the interaction between cells and Gel/PLCL nanofiber were evaluated by FESEM and fluorescent microscopy. MTS assay and quantitative real-time polymerase chain reaction were applied to assess the proliferation of co-cultured ADSs/HFCs and the collagen type I and III synthesis, respectively. The concentrations of two cytokines including fibroblast growth factor-basic and transforming growth factor-ß1 were also measured in culture medium of co-cultured ADSs/HDCs using enzyme-linked immunosorbent assay assay. Actually, nanofibers exhibited proper structural properties in terms of stability in cell proliferation and toxicity analysis processes. Gel/PLCL nanofiber promoted the growth and the adhesion of HFCs. Our results showed in contact co-culture of ADSs/HFCs on the Gel/PLCL nanofiber increased cellular adhesion and proliferation synergistically compared to non-coated plate. Also, synthesis of collagen and cytokines secretion of co-cultured ADSs/HFCs on Gel/PLCL scaffolds is significantly higher than non-coated plates. To conclude, the results suggest that Gel/PLCL nanofiber can imitate physiological characteristics in vivo and enhance the efficacy of co-cultured ADSs/HFCs in wound healing process.

Recent Advances in Natural Gum-Based Biomaterials for Tissue Engineering and Regenerative Medicine: A Review.

The engineering of tissues under a three-dimensional (3D) microenvironment is a great challenge and needs a suitable supporting biomaterial-based scaffold that may facilitate cell attachment, spreading, proliferation, migration, and differentiation for proper tissue regeneration or organ reconstruction. Polysaccharides as natural polymers promise great potential in the preparation of a three-dimensional artificial extracellular matrix (ECM) (i.e., hydrogel) via various processing methods and conditions. Natural polymers, especially gums, based upon hydrogel systems, provide similarities largely with the native ECM and excellent biological response. Here, we review the origin and physico-chemical characteristics of potentially used natural gums. In addition, various forms of scaffolds (e.g., nanofibrous, 3D printed-constructs) based on gums and their efficacy in 3D cell culture and various tissue regenerations such as bone, osteoarthritis and cartilage, skin/wound, retinal, neural, and other tissues are discussed. Finally, the advantages and limitations of natural gums are precisely described for future perspectives in tissue engineering and regenerative medicine in the concluding remarks.

A green method for the synthesis of pyrrole derivatives using arylglyoxals, 1,3-diketones and enaminoketones in water or water-ethanol mixture as solvent.

Three-component reaction between arylglyoxals, 1,3-dicetones and enaminoketones leads to new polyfunctionalized tetraone derivatives which may be easily converted to polyfunctionalized pyrroles. Reactions were conducted in water or water-ethanol mixture as green solvents, and all products were isolated by simple washing of the resulting solids with diethyl ether.

Detection of axillary perspiration metabolites using ion mobility spectrometry coupled to rapid gas chromatography.

The composition of human sweat-and as a consequence the composition of volatiles released from human skin-strongly depends on genetic preconditions, diet, stress, personal hygiene but also on health status and medication. Accordingly, the composition is a carrier of information on the physical and mental states of a person. Therefore, rapid on-site analysis of the relevant substances may be used for medical diagnosis and medication control or even for psychological characterisation. Ion mobility spectrometry coupled to rapid gas chromatography (GC-IMS) was applied to the analysis of human axillary sweat as a sensitive, selective, rapid, and non-invasive method in a feasibility study. For this purpose, a sampling chamber was designed and manufactured. The design and the experimental setup were validated successfully. At least 179 human metabolites could be detected by GC-IMS from the skin of 7 volunteers. Fifteen metabolites were available in all samples from all volunteers and therefore can be characterised as basic sweat compounds which might enable the localisation of hidden persons. Furthermore, in a preliminary feasibility study, the potential of GC-IMS for differentiating the composition of sweat after physical exercises and in a stressful situation-even gender specific-could be demonstrated. Thus, with GC-IMS, a rapid and mobile analytical tool for the analysis of skin volatiles is available for a broad range of applications, e.g. with regard to axillary odour, human health, nutrition, consumption of remedies or drugs of abuse, the localisation of trapped or hidden persons, or even the characterisation of the reaction on stressful situations. Graphical abstract.

Low cost hydrogels based on gum Tragacanth and TiO2 nanoparticles: characterization and RBFNN modelling of methylene blue dye removal.

In this study, low-cost and high adsorption capacity hydrogels based on gum tragacanth biopolymer (GT or TG) and TiO2 nanoparticles were produced by using glutaraldehyde as the crosslinking agent. These hydrogels were applied in photocatalytic process to remove methylene blue from simulated colored solution. TiO2-Gum Tragacanth hydrogels (TGTH) were characterized by FESEM and FTIR to investigate the surface morphology and functional group of the synthesized hydrogel. Contact angle measurements showed that, the hydrophilicity nature of crosslinked TGTH decreased compared to GT films. The effect of particle size, initial dye concentration, pH of the solution and adsorbent/photocatalyst dosage on the removal efficiency was assessed. The obtained results demonstrated that lower dosage of the prepared TGTH (0.15g/L) outperformed GT (0.2 g/L) reaching 87% of dye removal, while GT resulted in 69% of removal. In order to model the cationic dye removal process, a Radial Basis Function Neural Network (RBFNN) was investigated. This network was applied to predict dye removal based on the time duration, initial dye concentration, pH of the solution and TiO2dosage in gum tragacanth hydrogel structure ([TiO2/gum tragacanth hydrogel]0 (g/L)). The performance of the proposed model was validated by several training data. The RBFNN model mostly overlapped with the experimental data due to selecting proper structure and training algorithm.

Preparation and optimization photocatalytic activity of polymer-grafted Ag@AgO core-shell quantum dots.

A linear-dendric copolymer containing polyethylene glycol-polycitric acid used as a capping agent to the green inter-matrix synthesis of silver/silver oxide core-shell quantum dots (Ag@AgO QDs). Water-soluble Ag@AgO QDs were synthesized with high yield and narrow size distribution. Here, Ag ions were trapped in the polymer branches and covalently bonded to it. Another sample of Ag@AgO QDs was synthesized through the same method and conditions without any capping agent (raw nanoparticles). Structure, size distribution, and morphology of raw and copolymer-grafted nanoparticles were identified using X-Ray diffraction, field emission scanning electron microscopy (FESEM), and high-resolution transmission electron microscopy (HRTEM). The results from XRD pattern and UV spectra confirmed the Ag@AgO structure of both nanoparticles. From the FESEM image, the size of Ag nanoparticles obtained at the range of 1-20 nm. HRTEM image of grafted nanoparticles directly showed that these nanoparticles have very tiny size in the range of 1-2 nm and presented in the form of core-shell Ag@AgO. Thus, both raw and polymer-grafted samples are in the range of quantum dots (QDs). Raw and polymer-grafted Ag@AgO QDs which take the advantage of water solubility and biosafety, were used as photocatalyst for degradation of cationic methylene blue (MB) and anionic methyl orange (MO) dyes at low and high concentrations of each dye. Results shows using polymer-grafted QDs leads to a significant enhancement both in the efficiency and rate of dye degradation, compared to the case of using raw nanoparticles.

Development of biodegradable electrospun gelatin/aloe-vera/poly(ε­caprolactone) hybrid nanofibrous scaffold for application as skin substitutes.

Nowadays, aloe-vera (AV) is exploited extensively in nanofibrous structures for skin substitutes. However, the lack of electrospinnability and appropriate mechanical characteristics are the key limitations for this natural extract to be used in the form of nanofibrous mats. In this study, two commercially available biopolymers, gelatin (Gel) and poly(ε­caprolactone) (PCL), were chosen to improve these issues and double-nozzle electrospinning technique was used to fabricate hybrid scaffold from Gel/AV blend and PCL solutions. Response surface methodology was utilized to investigate the effect of electrospinning parameters (Gelatin concentration, Aloe-vera concentration and Gel/AV feed-rate) on the mechanical properties, morphology and hydrophilicity of nanofibers and the optimized scaffold was chosen for further studies. In order to verify the application of this scaffold in bioapplications, the chemical, thermal and biological features of scaffold were analyzed using FTIR, DSC, biodegradability, bactericidal, biocompatibility and drug-delivery. The results revealed that the presence of aloe-vera improved the antibacterial activity (>99% and 85.63% against Gram-positive and Gram-negative bacteria, respectively), and led to adequate in-vitro biodegradation. Furthermore, it was found that incorporation of aloe-vera increased the cell viability without any toxicity.

Production of cotton fabrics with durable antibacterial property by using gum tragacanth and silver.

A simple and inexpensive procedure has been devised to prepare antibacterial cotton fabric using silver nanoparticles (AgNPs) and bio degradable gum tragacanth (GT). For this, different concentrations of GT (2, 4 and 6 g/L) along with a constant amount of Ag (5%; according to weight of dry GT used in the solutions) were applied to investigate the efficacy of antibacterial potency against Escherichia coli and Staphylococcus aureus and their effect on physical, mechanical and biological characteristics of cotton fabric. Our study exhibited the presence of small amount of AgNPs in the composite structure was enough to increase the antibacterial activity of fabrics compared to fabric that were treated by only GT. Moreover, the treated cotton with GT-4%/Ag indicated proper tensile strength and stiffness compared to treated fabric with GT-6%/Ag composite. The biocompatibility of the GT and GT/Ag treated fabrics was verified through MTT assay on fibroblast cells.

Fabrication, optimization and characterization of electrospun poly(caprolactone)/gelatin/graphene nanofibrous mats.

Recently graphene-based materials have been exploited widely in graphene-polymer nanocomposites and hold notable potential for various applications. In this study novel graphene-incorporated poly(caprolactone)/gelatin nanofibrous web were produced by electrospinning technique using acetic acid as a cost-effective eco-friendly solvent. Response surface methodology was used for optimizing the diameter of the electrospun nanofibrous web. To tailor electrospun nanofibers with suitable mechanical and electrical properties, the impact of affecting electrospinning parameters was studied. Our results show that, with increasing the PCL/gelatin ratio, the diameter of nanofibers increases, whereas increasing graphene concentration decreased the diameter of nanofibers up to an optimum content. With the incorporation of 1.5% graphene into PCL/gelatin matrix the tensile strength and Young modulus of nanofibrous mat considerably increased by 117 and 128% respectively. The electrical conductivity results demonstrated that nanofibrous mats own nearly 11 times higher conductivity than that of PCL/gelatin nanofibers when the graphene concentration reached the percolation threshold. Contact angle measurements confirmed that graphene-incorporated electrospun nanofibers were more hydrophilic than that of neat nanofibrous mats. Cellular toxicity results of electrospun nanofibers ascertained almost no toxicity to PC12 cells. The morphology of electrospun nanofibers was investigated by means of scanning electron microscopy (SEM). FTIR and DSC analysis revealed that there might be possible interactions between graphene and PCL/gelatin matrix. XRD analysis demonstrated that graphene-incorporated PCL/gelatin nanofibers exhibited higher crystallinity. The uniform dispersion of graphene nanosheets in nanofibrous mat was also verified through Raman spectroscopy.

Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers.

In this study we describe the potential of electrospun curcumin-loaded poly(ε-caprolactone) (PCL)/gum tragacanth (GT) (PCL/GT/Cur) nanofibers for wound healing in diabetic rats. These scaffolds with antibacterial property against methicillin resistant Staphylococcus aureus as gram positive bacteria and extended spectrum ß lactamase as gram negative bacteria were applied in two forms of acellular and cell-seeded for assessing their capability in healing full thickness wound on the dorsum of rats. After 15days, pathological study showed that the application of GT/PCL/Cur nanofibers caused markedly fast wound closure with well-formed granulation tissue dominated by fibroblast proliferation, collagen deposition, complete early regenerated epithelial layer and formation of sweat glands and hair follicles. No such appendage formation was observed in the untreated controls during this duration. Masson's trichrome staining confirmed the increased presence of collagen in the dermis of the nanofiber treated wounds on day 5 and 15, while the control wounds were largely devoid of collagen on day 5 and exhibited less collagen amount on day 15. Quantification analysis of scaffolds on day 5 confirmed that, tissue engineered scaffolds with increased amount of angiogenesis number, granulation tissue area (µ(2)), fibroblast number, and decreased epithelial gap (µ) can be more effective compared to GT/PCL/Cur nanofibers.

Gum tragacanth/poly(l-lactic acid) nanofibrous scaffolds for application in regeneration of peripheral nerve damage.

Nanofibrous nerve guides have gained huge interest in supporting the peripheral nerve regeneration due to their abilities to simulate the topography, mechanical, biological and extracellular matrix morphology of native tissue. Gum tragacanth (GT) is a biocompatible mixture of polysaccharides that has been used in biomedical applications. During this study, we fabricated aligned and random nanofibers from poly(l-lactic acid) and gum tragacanth (PLLA/GT) in various ratios (100:0, 75:25, and 50:50) by electrospinning. Scanning electron microscope demonstrated smooth and uniform nanofibers with diameters in the range of 733±65nm and 226±73nm for align PLLA and random PLLA/GT 50:50 nanofibers, respectively. FTIR analysis, contact angle, in vitro biodegradation and tensile measurements were carried out to evaluate the chemical and mechanical properties of the different scaffolds. PLLA/GT 75:25 exhibited the most balanced properties compared to other scaffolds and was used for in vitro culture of nerve cells (PC12) to assess the potential of using these scaffolds as a substrate for nerve regeneration. The cells were found to attach and proliferate on aligned PLLA/GT 75:25 scaffolds, expressing bi-polar neurite extensions and the orientation of nerve cells was along the direction of the fiber alignment. Results of 8 days of in vitro culture of PC12 cells on aligned PLLA/GT 75:25 nanofibers, showed 20% increase in cell proliferation compared to PLLA/GT 75:25 random nanofibers. PLLA/GT 75:25 aligned nanofibers acted as a favorable cue to support neurite outgrowth and nerve cell elongation compared with PLLA nanofibers. Our results showed that aligned PLLA/GT 75:25 nanofibers are promising substrates for application as bioengineered grafts for nerve tissue regeneration.

Electrospinning of PLGA/gum tragacanth nanofibers containing tetracycline hydrochloride for periodontal regeneration.

Controlled drug release is a process in which a predetermined amount of drug is released for longer period of time, ranging from days to months, in a controlled manner. In this study, novel drug delivery devices were fabricated via blend electrospinning and coaxial electrospinning using poly lactic glycolic acid (PLGA), gum tragacanth (GT) and tetracycline hydrochloride (TCH) as a hydrophilic model drug in different compositions and their performance as a drug carrier scaffold was evaluated. Scanning electron microscopy (SEM) results showed that fabricated PLGA, blend PLGA/GT and core shell PLGA/GT nanofibers had a smooth and bead-less morphology with the diameter ranging from 180 to 460 nm. Drug release studies showed that both the fraction of GT within blend nanofibers and the core-shell structure can effectively control TCH release rate from the nanofibrous membranes. By incorporation of TCH into core-shell nanofibers, drug release was sustained for 75 days with only 19% of burst release within the first 2h. The prolonged drug release, together with proven biocompatibility, antibacterial and mechanical properties of drug loaded core shell nanofibers make them a promising candidate to be used as drug delivery system for periodontal diseases.

Electrospun curcumin loaded poly(ε-caprolactone)/gum tragacanth nanofibers for biomedical application.

In this work curcumin (Cur)-loaded poly(ε-caprolactone) (PCL)/gum tragacanth (GT) scaffold membranes which provided the controlled release of curcumin for over 20 days were fabricated by electrospinning. Field Emission Scanning Electron Microscopy (FESEM) analysis, Fourier Transform Infrared Spectroscopy (FTIR) and differential scanning calorimetry (DSC) were applied to characterize the produced nanofibers. These nanofibers were evaluated for water absorption capacity, in vitro drug release, biodegradation test, cell culture and MTT analysis. The water contact angle measurements indicated that addition of GT and curcumin in composition resulted in increase in the hydrophilicity of the nanofibers. Biodegradation test for the fabricated nanofibers exhibited that PCL/GT, PCL/Cur-3% and PCL/GT/Cur-3% nanofibers preserved their structure after 15 days. The in vitro release profile of curcumin showed 6.86, 14 and 30.09% burst release for PCL/GT/Cur-1%, PCL/GT/Cur-3% and PCL/Cur-3% nanofibers respectively. The effect of curcumin concentration in the nanofibers composition on the cell viability was assessed by the MTS assay. The cytotoxic effect of released curcumin on the fibroblast cells was examined. The PCL/GT/Cur-3% with suitable mechanical properties, excellent biological characteristics, and maintaining their original structure in degradation media may have potential application as a wound dressing patch for healing slow rate wounds.